So I did a little googling after my first reply. It could possibly be because of system restore, actually. I have had this problem before, somehow system restore decided to allocate over 80GB of space to restore points. I have not tested yet whether or not this is actually the cause, but it might be a good theory.
Windows Vista. Gotta love it.
"2. After you run Defraggler in Windows Vista, there is less free hard drive space available.
When you run certain third-party programs (such as Defraggler), Windows Vista creates an automatic System Restore point for security reasons. This System Restore point takes up hard drive space. See this topic for more information on Windows Vista and shadow copies."