All in the title
Yes.
About 1 year ago I got a new USB3 stick and was trying to find if cluster size effected speed in any discernible way.
I was reformatting it with FAT, NTFS, 4k, 8k, 16k etc clusters and found DF had no issues.
Yes, OK , I had a doubt because It was impossible for me to defrag a 120 Go NTFS 8k cluster size formated partition. It was first fragmented to 53 % with 24% free space = no way, but with 32% fragmentation and 57 % free space it was not better but though it was conscensciously working as if it wanted to perform the task for hours ....
I had finally to empty that partition
I profit the occasion to format a 4 Go partition and make transfer tests on it with different cluster sizes and between 512 o and 64ko I did not see any difference what ever the size of the files tested from 4 ko to 512 ko or bit to bit
then I reformated it to 8 k
I ended up going back to 4K (from memory) as I found no benefit either.
If you knew you were only going to move large files, or only small files, then you could have the appropriate cluster size to match, but my file sizes at least are too variable to notice any performance boost.