Updating from 1.21 to 2.0 under Win7 64bit, running interactively the "Move Large File" option works as expected with Minimum size set to 100MB.
Have noticed a 2.0 scheduled defrag, re-located all the large files from end of drive C:, re-running defrag interactively shifted them back as expected as per the options. There is one file over the 250MB default for minimum size in C: and it was moved to (C:\Windows\Installer\19230e-4.msp)