Recover from External HDD-FAT32 using Win10- with NTFS

Will Recuva recover audio/video files fro an HDD in a sled that is formatted in FAT32. I am using Win10 with NTFS. Without additional software, just MS File Explorer, I get a message that I do not have permission to access that drive and the tab to change permissions does not appear. This drive was a backup drive used in previous computers.

Recuva will recover candidate files from FAT32 to NTFS and vice-versa. However this does not necessarily mean that you will find the files you are looking for, or that they will be in a recoverable state. You must be able to access the source disk by drive letter in Explorer with the relevant permissions.