Major problems recovering images from separate HD

Can any one help?

I have attempted to recover pictures from back up file on a separate Hard Drive. It found all the pictures in a backup recycle bin folder and attempted to recover them in a new folder on the desktop. It has copied the files bit with 0 bytes and now they have disappeared off the hard drive now when conducting a new scan. Have i lost them now permanently? has the pc overwritten them?

Help me Obi One!