After the Anniversary Update 2016 to Windows 10 Home Edition I have used the Registry Cleaner in CCleaner to clean the registry, and now Windows Defender Offline has stopped working. Nothing at alle happens, when I use the Defender Offline-button
try reloading the backed up .reg file created by CC (if you said Yes to the prompt)
but I would be holding off using the reg cleaning portion of CC until the next CC release.
Win10 build 14393 has only just been released and CC may not handle any specific changes to whatever MS may have changed in the registry.
I do know MS has giving Defender an overhaul at least.